ETD

Response to Diurnally-Varying Environmental Stressors in Larvae of a Marine Gastropod

Public Deposited

Many marine organisms grow shells or skeletons that are made of calcium carbonate. The
fluorescent dye, calcein, labels calcium carbonate in actively growing shells and has been used to
tag mollusc larvae for studies of larval dispersal. We developed calcein labeling as a tool to
study short term variation in growth responses to rapidly changing environmental conditions. We
first explored the effect of calcein labeling on growth in larvae of Crepidula fornicata, a
gastropod mollusc that has been widely used in studies of larval ecology. Four-day-old larvae
were treated with calcein (20 mg/L) for 4 or 12 hours, and their growth was compared to
untreated controls for 8 days. Shell growth rates and bright label bands were equivalent in all
three groups. Calcein labeling did not have detrimental effects on larval shell growth, and can
mark growth periods as short as 4 hours. These results led to a second experiment in which shell
growth increments were differentially labeled to determine the diurnal timing of growth relative
to food availability during the day versus at night. This led to during both day and night feeding
treatments, maximum growth occurred during the 12 hr period following the feeding, rather than
during the feeding period itself. After successfully marking short periods of larval growth an
experiment in which the combined effects of ocean acidification and oxygen depletion compared
to individual stressor effects was performed using calcein labeling method. In order to test which
oxygen depletion level was most beneficial to the experiment a hypoxic toxicity experiment was
done, after five days under treatment the hypoxic treatment was 11% smaller. The combined
stressor experiment led to unexpected results of opposite additive effects, the larvae with double
stressor OA/Hypoxia in fact grew 27% larger than the control group. This could mean that larvae
rebound after stressful conditions and put more focus on growth when put under control
conditions.


MLA citation style (9th ed.)

Almanzar, Dianny Aalyah. Response to Diurnally-varying Environmental Stressors In Larvae of a Marine Gastropod. . 2022. dickinson.hykucommons.org/concern/etds/df02b649-e337-46df-94e1-f616f0b9f9d7?locale=en.

APA citation style (7th ed.)

A. D. Aalyah. (2022). Response to Diurnally-Varying Environmental Stressors in Larvae of a Marine Gastropod. https://dickinson.hykucommons.org/concern/etds/df02b649-e337-46df-94e1-f616f0b9f9d7?locale=en

Chicago citation style (CMOS 17, author-date)

Almanzar, Dianny Aalyah. Response to Diurnally-Varying Environmental Stressors In Larvae of a Marine Gastropod. 2022. https://dickinson.hykucommons.org/concern/etds/df02b649-e337-46df-94e1-f616f0b9f9d7?locale=en.

Note: These citations are programmatically generated and may be incomplete.

Relations

In Collection: